|
KMID : 0616120070380040339
|
|
Korean Journal of Pharmacognosy
2007 Volume.38 No. 4 p.339 ~ p.348
|
|
|
Inhibition of LPS Induced iNOS, COX-2 and Cytokines Expression by Genistein-4’-O-α-L-Rhamnopyranosyl-(1-2)-β-D-Glucopyranoside through the NF-κB Inactivation in RAW 264.7 Cells
|
|
Park Seung-Jae
Lee Kyung-Tae
Baek Nam-In
Cho Young-Wuk
Ahn Eun-Mi
Kim Ji-Yeon
Jang Young-Pyo
|
|
|
Abstract
|
|
|
This study were designed to evaluate the anti-inflammatory effects of genistein-4’-O-α-L-rhamnopyranosyl-(1-2)-β-D-glucopyranoside (GRG) isolated from Sophora japonica (Leguminosae) on the lipopolysaccharide (LPS)-induced nitric
oxide (NO) and prostaglandin (PGE2) production by RAW 264.7 cell line. GRG significantly inhibited the LPS-induced NO and PGE2 production. Consistent with these observations, GRG reduced the LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein and mRNA levels in a concentration-dependent manner. In addition, the release and the mRNA expression levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were also reduced by GRG. Moreover, GRG attenuated the LPS-induced activation of nuclear factor-kappa B (NF-κB), a transcription factor necessary for pro-inflammatory mediators, iNOS, COX-2, TNF-α and IL-6 expression. These results suggest that the down regulation of iNOS, COX-2, TNF-α, and IL-6 expression by GRG are achieved by the downregulation of NF-κB activity, and that is also responsible for its anti-inflammatory effects.
|
|
|
KEYWORD
|
|
|
genistein-4’-O-α-L-rhamnopyranosyl-(1-2)-β-D-glucopyranoside, NF-κB, LPS, Anti-inflammation, Sophora
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|